IN SITU SAMPLE PREPARATION AND DETERMINATION OF DAPSONE IN TABLET FORMULATION USINGA NEW CHEMICAL MICRO-TOTAL ANALYSIS SYSTEM WITHOUT MICROCHIPS

Abstract

In this work, a novel study was investigated in situ sample preparation using the green micro total analysis system
(μTAS) without microchips for the determination of dapsone. The microchips have been replaced by capillary tubes with
very small internal diameters. These capillary tubes are used in the field of human medicine. As a spectrophotometric reagent, 7-chloro-4-nitrobenzoxadiazole (NBD-Cl) was employed. The suggested approach was dependent on the desired medication, NBD-Cl, reacting in an alkali solution to produce a coloured adduct that had λmax = 468 nm. Investigations have been done into the several physical and chemical factors that affect the measurement, including sample volume, pH, flow rate, the reacting loop, and the volume and amount of NBD-Cl. This method shows the linearity range of 0.5 to 100 μg mL-1 with a coefficient of regression R2 of 0.9989. The regression equation A = 0.0697C + 0.0034. Also, the limit of detection was 0.3998μg mL-1. The proposed system has been applied
efficaciously to determine the presence of dapsone in medical preparations.