APPLICATION OF AN ION PAIR ASSOCIATION COMPLEX FORMATION REACTION FOR THE ESTIMATION OF ACE INHIBITOR IN FORMULATIONS

Abstract

Novel and sensitive analytical technique was proposed for estimating perindopril (angiotensin-converting enzyme (ACE) inhibitor) in formulations using acidic dye as chromogenic reagent. The study was established on the development of colour species formation between perindopril erbumine and chromogenic reagent such as azocarmine G (ACG). Proposed method consists formation of an ion-pair association complex with azocarmine-G. The optical density was measured at lmax of 540 nm. Regression analysis of proposed method showed that the linearity lies within the concentration limits of 20-100 μg mL^-1. Molar absorptivity (εmax) values of developed method are found to be as 3.22 × 103 L mol^-1 cm^-1. Investigated method was found to have linear correlation coefficient (r) value of 0.9999. Proposed method obeyed Lambert-Beer’ law with reproducible results. Percentage recovery was found to be within the limits of 99.7 - 100.1 (± 0.41 SD, ± 0.57 SD). All the parameters were validated statistically.