GREEN SYNTHESIS OF GOLD NANOPARTICLES USING MENTHA SPICATA L. AND ITS APPLICATION AS A CATALYST FOR OXIDATION NADH TO NAD+
DOI:
https://doi.org/10.59957/jctm.v61.i3.2026.5Keywords:
nanotechnology, fluorescence, nicotinamide adenine dinucleotide, gold nanoparticles, antioxidantAbstract
Due to their high biocompatibility, stability, and unique characteristics, gold nanoparticles (AuNPs) have numerous applications in the medical and biological fields. Plant-based nanoparticle synthesis is beneficial over other biological processes because it can be scaled up to an industrial scale. The electrochemical measurement of dihydronicotinamide adenine dinucleotide (NADH) is crucial because it serves as a required coenzyme for several dehydrogenases. NADH is the final electron donor molecule in the mitochondrial electron transport pathway. This study examined the catalytic role of AuNPs produced using Mentha spicata L. in the oxidation of NADH to
nicotinamide adenine dinucleotide (NAD+). AuNPs reduced NADH fluorescence intensity. As the quantity of AuNPs increased, the intensity of the 260 nm NAD+ band increased, while that of the 340 nm NADH band decreased. The surface plasmon absorbance band of AuNPs at 520 nm increased in intensity due to the presence of NADH. This study provides significant evidence that the AuNPs surface catalyzes the oxidation of NADH to NAD+. The catalyst characteristic of this crucial reaction may have significant potential applications in the biological and medicinal fields.
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