TRACE ANALYSIS OF BRILLIANT GREEN DYE BY FLOW INJECTION TECHNIQUE

Abstract

A low-cost and high throughput flow injection analysis (FIA) system design is conducted to determine Brilliant Green (BG) dye. The research includes studying the chemical and physical variables and control of optimal conditions of the FIA system. The merging zone technique uses a homemade flow injection valve to determine BG dye. The optimum conditions are studied, such as the flow rate of the carrier, optimal dye volume, repeatability, dead volume, and dispersion coefficient. The flow rate is 3.0 mL min^-1, the optimal volume of dye is 82.425 μL, the repeatability is high for eight measurements (n = 8), and the values of dispersion coefficient are 1.41 and 1.44 for the concentrations of 4 and 7 mg L^-1, respectively. The calibration graphs have linear ranges of (0.5 - 10) mg L^-1 and (0.07 - 16) mg L^-1, with detection limits of 0.413 and 0.068 mg L^-1 for the spectrophotometric and FIA methods, respectively. The results obtained were statistically treated. FIA method successfully applied to standard solutions demonstrates its reliability and potential for further applications.