PRODUCTION, PHYSICOCHEMICAL AND THERMODYNAMIC CHARACTERIZATION OF BIOETHANOL DERIVED FROM CORK: A PROMISING VALORIZATION PATHWAY FOR A NON-FOOD LIGNOCELLULOSIC WASTE
DOI:
https://doi.org/10.59957/jctm.v61.i2.2026.13Keywords:
Bioethanol, cork biomass, lignocellulosic waste, alcoholic fermentation, physicochemical and thermodynamic caracteristicsAbstract
This study investigates the potential of cork biomass, an underutilized lignocellulosic residue, to produce second-generation bioethanol. The process involved biomass preparation, Thermochemical pretreatment, concentrated acid hydrolysis, and alcoholic fermentation to convert the carbohydrate fraction of cork into fermentable sugars. The resulting bioethanol, obtained through distillation, reached a purity of 30 % (v/v). Physicochemical and thermodynamic caracteristics of the produced bioethanol included measurements of density, viscosity, flash point, calorific value, and vapor pressure. Spectroscopic analyses (FT-IR and Raman) were also conducted to confirm ethanol composition and purity. Despite its moderate purity, the bioethanol exhibited properties in line with initial fermentation - stage fuels, confirming the feasibility of cork as a raw material for biofuel production. These findings demonstrate a promising valorization route for cork waste within the framework of circular bioeconomy and sustainable energy development.
References
S. Periyasamy, J. Beula Isabel, S. Kavitha, V. Karthik, B. A. Mohamed, D. G. Gizaw, P. Sivashanmugam, T. M. Aminabhavi, Recent advances in consolidated bioprocessing for conversion of lignocellulosic biomass into bioethanol - a review, Chem. Eng. J., 453,1, 2023, 139783.
G. Baskar, S. J. Pandian, S. M. R. Reddy, K. S. T. Ramya, R. Naveenkumar, R. Pravin, Utilization of municipal solid waste for bioethanol production using Trichoderma reesei and Saccharomyces cerevisiae, J. Energy Environ. Sustain., 13–14, 2023, 14 17.
W.H. Wu, W.C. Hung, K.Y. Lo, Y.H. Chen, H.P. Wan, K.C. Cheng, Bioethanol production from taro waste using thermo tolerant yeast Kluyveromyces marxianus K21, Bioresour. Technol., 201, 2016, 27 32.
S. Niphadkar, P. Bagade, S. Ahmed, Bioethanol production: insight into past, present and future perspectives, Biofuels, 9, 2018, 229 238.
T. Ivanov, S. Petrin, I. Lalov, Ryegrass as a feedstock for bioethanol production, J. Chem. Technol. Metall., 58, 5, 2023, 840 844.
O. Awogbemi, D. V. V. Kallon, Valorization of agricultural wastes for biofuel applications, Heliyon, 8, 2, 2022, e11117.
M. Broda, D. J. Yelle, K. Serwańska, Bioethanol production from lignocellulosic biomass – challenges and solutions, Molecules, 27, 24, 2022, 8717.
Z. Anwar, M. Gulfraz, M. Irshad, Agro industrial lignocellulosic biomass: a key to unlock the future bio energy - a brief review, J. Radiat. Res. Appl. Sci., 7, 2014, 2, 163 173.
A. H. Alabdalall, A. A. Almutari, S. A. Aldakeel, L.A.; Alsoufi, M.H.; Alfuraih, L.Y.; Elkomy, H.M, Bioethanol production from lignocellulosic biomass using Aspergillus niger and A. flavus hydrolysis enzymes through immobilized S. cerevisiae, Energies, 16, 2, 2023, 823.
N. A. Buijs, V. Siewers, J. Nielsen, Advanced biofuel production by the yeast Saccharomyces cerevisiae, Curr. Opin. Chem. Biol., 17, 3, 2013, 480 488.
S. A. Jambo, R. Abdulla, S. H. Mohd Azhar, H. Marbawi, J, A review on third generation bioethanol feedstock, Renew. Sustain. Energy Rev., 65 C, 2016, 756 769.
P. V. Neves, A. P. Pitarelo, L. P. Ramos, Production of cellulosic ethanol from sugarcane bagasse by steam explosion: effect of extractives content, acid catalysis and different fermentation technologies, Bioresour. Technol., 208, 2016, 184 194.
H. Zhang, L. Li, Unpolluted fractionation of wheat straw by steam explosion and ethanol extraction, Bioresour. Technol., 98, 3, 2007, 666 676.
B. C. Saha, L. B. Iten, M. A. Cotta, Y. V. Wu, Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol, Biotechnol. Prog., 21,3, 2005, 816 822.
E. I. Melekwe, S. A. Lateef, G. Rowland, E. Ekpeyong, Bioethanol production potentials of corn cob, waste office paper and leaf of Thaumatococcus daniellii, curr. J. Appl. Sci. Technol., 17, 4, 2016, 1 10.
Z. Li, P.R. Waghmare, L. Dijkhuizen, X. Meng, W. Liu, Research advances on the consolidated bioprocessing of lignocellulosic biomass. Eng. Microbiol., 2024, 4, 100139.
P. Sharma, N. Sharma, RSM approach to pre-treatment of lignocellulosic waste and a statistical methodology for optimizing bioethanol production. Waste Manag Bull., 2024, 2, 1, 49–66.
Y. Chen, H. Yang, H. Zou, T. Sun, M. Li, J. Zhai, Q. He, L. Gu, W.Z. Tang, Effects of acid/alkali pretreatments on lignocellulosic biomass mono-digestion and its co-digestion with waste activated sludge, J. Clean. Prod., 2020, 277, 123471.
P.A. Ramirez-Cabrera, J.J. Lozada-Castro, C.A. Guerrero-Fajardo, Screw reactor design for potato peel pretreatment using the steam explosion, Bioresour. Technol., 2024, 400, 130675.
A. Woźniak, K. Kuligowski, L. Świerczek, A. Cenian, Review of lignocellulosic biomass pretreatment using physical, thermal and chemical methods for higher yields in bioethanol production, Sustainability, 2025, 17, 1, 287.
A. Topaloglu, Ö. Esen, B. Turanlı-Yıldız, M. Arslan, Z.P. Çakar, From Saccharomyces cerevisiae to ethanol: unlocking the power of evolutionary engineering in metabolic engineering applications, J. Fungi., 2023, 9, 984-1026.
K.N. Tsegaye, M. Alemnew, N. Berhane, Saccharomyces cerevisiae for lignocellulosic ethanol production: a look at key attributes and genome shuffling, Front. Bioeng. Biotechnol., 2024, 12,1466644.
A. Limayem, S.C. Ricke,. Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects, Prog. Energy Combust. Sci., 2012, 38, 4, 449-467.
H. Pereira, Cork: Biology, Production and Uses, Amsterdam, Elsevier; 2007.
P. Boudy, North african forest economy: monographs and treatments of forest species, Paris, Larose, 1950 (Economie forestière nord-africaine: Monographies des forêts types)
S. Beluhan, K. Mihajlovski, B. Šantek, M. Ivančić Šantek, The production of bioethanol from lignocellulosic biomass: pretreatment methods, fermentation, and downstream processing. Energies, 2023, 16, 19, 700.
C.W. Dence, in: S.Y. Lin, C.W. Dence, (Eds.), Methods in Lignin Chemistry, Heidelberg: Springer-Verlag, 1992, p. 33-61.
L.E. Wise, M. Murphy, A.A. D'Addieco, Chlorite holocellulose, its fractionation and bearing on summative wood analysis and studies on the hemicelluloses. Pap. Trade. J., 1946, 122, 2, 35-43.
P. Alvira, E. Tomás-Pejó, M. Ballesteros, M.J. Negro, Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review, Bioresour. Technol., 2010, 101, 13, 4851-4861.
C. Cara, E. Ruiz, I. Ballesteros, M.J. Negro, E. Castro, Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline peroxide delignification, Process Biochem., 2006, 41, 423-429.
S. Beluhan, K. Mihajlovski, B. Šantek, M. Ivančić Šantek, The production of bioethanol from lignocellulosic biomass: pretreatment methods, fermentation, and downstream processing, Energies, 2023, 16, 19, 7003.
Y. Sun, J. Cheng, Hydrolysis of lignocellulosic materials for ethanol production: a review, Bioresour. Technol., 2002, 83, 1, 1-11.
M.J. Taherzadeh, K. Karimi, Acid based hydrolysis processes for ethanol from lignocellulosic materials: a review, BioResources, 2007, 2, 3, 472-499.
P. Kumar, D.M. Barrett, M. J. E. Delw Palmqvist, P. Stroeve, Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production, Ind. Eng. Chem. Res., 2009, 48, 8, 3713-3729.
N. Mosier, C. Wyman, B. Dale, R. Elander, Y. Y. Lee, M. Holtzapple, M. Ladisch, Features of promising technologies for pretreatment of lignocellulosic biomass, Bioresour. Technol., 2005, 96, 6, 673-686.
J. R. M. Almeida, M. Bertilsson, M.F. Gorwa Grauslund, S. Gorsich, G. Lidén, Metabolic effects of furaldehydes and impacts on biotechnological processes, Appl. Microbiol. Biotechnol., 2007, 82, 4, 625-638.
A. Demirbaş, Biomass resource facilities and biomass conversion processing for fuels and chemicals, Energy. Convers. Manag., 2001, 42, 11, 1357-1378.
S.S. Rahman, M. M. Hossain, N. Choudhury, Effect of various parameters on the growth and ethanol production by yeasts isolated from natural sources, Bangladesh. J. Microbiol., 2013, 30, 1-2, 49-54.
N.M. Tripathi, G.K. Aseri, A.K. Jain, D. Sharma, D. Singh, Comparative ethanol production by Saccharomyces cerevisiae and Saccharomyces bayanus using apple juice concentrate, Microbial. Biosyst. 2025, 10, 2, 322469.
D.P. Procópio, J.W. Lee, J. Shin, R. Tramontina, P.F. Ávila, L.B. Brenelli, F.M. Squina, A. Damasio, S. C. Rabelo, R. Goldbeck, T. T. Franco, D. J. Leak, Y. S. Jin, T. O. Basso, Metabolic engineering of Saccharomyces cerevisiae for second generation ethanol production from xylo oligosaccharides and acetate, Sci. Rep., 2023, 13, 1, 19182.
M. van Dijk, F. Mierke, Y. Nygård, L. Olsson, Nutrient supplemented propagation of Saccharomyces cerevisiae improves its lignocellulose fermentation ability, AMB Express., 2020, 10, 1, 157.
Y. Yang, M. Hu, Y. Tang, B. Geng, M. Qiu, Q. He, S. Chen, X. Wang, S. Yang, Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis, Bioresour. Bioprocess., 2018, 5, 6, 1-12.
L.J. Jönsson, B. Alriksson, N. O. Nilvebrant, Bioconversion of lignocellulose: inhibitors and detoxification, Biotechnol. Biofuels., 2013, 6, 16.
M. J. Taherzadeh, K. Karimi, Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review, Int. J. Mol. Sci., 2008, 9, 9, 1621-1651.
I.M. Banat, P. Nigam, D. Singh, R. Marchant, A.P. McHale, Ethanol production at elevated temperatures and alcohol concentrations: part I - yeasts in general, World. J. Microbiol. Biotechnol., 1998, 14, 6, 809-821.
A. Boulal, B. Benali, M. Moulai, A. Touzi, Transformation of date waste from the Adrar region into bioethanol, Rev. Energies. Renouv., 2010, 13, 3, 455-463. (Transformation des déchets de dattes de la région d'Adrar en bioéthanol)
T. Masuko, A. Minami, N. Iwasaki, T. Majima, S. I. Nishimura, Y. C. Lee, Carbohydrate analysis by a phenol–sulfuric acid method in microplate format, Anal. Biochem., 2005, 339, 1, 69-72.
M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, F. Smith, Colorimetric method for determination of sugars and related substances, Anal. Chem., 1956, 28, 3, 350-356.
I. Mehani, B. Bouchekima, S. E. Laouini, M. Mehani, Production of bioethanol from waste dates in South Algeria: study and application, Res. J. Pharm. Technol., 2018, 11, 12, 5359-5365.
D. Neiva, H. Pereira, I. Miranda, R. Simões, Chemical depolymerization of cork suberin with hydrothermal processes, Ind. Crops. Prod., 2024, 208, 5.
L. Negahdar, I. Delidovich, R. Palkovits, Aqueous-phase hydrolysis of cellulose and hemicelluloses over molecular acidic catalysts: Insights into the kinetics and reaction mechanism, Applied Catalysis B: Environmental, 2016,184, 285-298.
E. Palmqvist, B. Hahn-Hägerdal, Fermentation of lignocellulosic hydrolysates. II: Inhibitors and mechanisms of inhibition, Bioresour. Technol., 2000, 74, 1, 25-33.
F. Alfani, A. Gallifuoco, A, Saporosi, A. Spera, A, M. Cantarella, Comparison of SHF and SSF processes for the production of ethanol from steam-exploded wheat straw, J. Ind Micro. Biotechnol., 2000, 25, 4, 184-192.
Y. Lin, S. Tanaka, Ethanol fermentation from biomass resources: Current state and prospects, App. Micro. Biotechnol., 2006, 69, 6, 627- 642.
A. Matsushika, H. Inoue, T. Kodaki, S. Sawayama, Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: Current state and perspectives, App. Micro.Biotechnol., 2009, 84, 1, 37-53.
B. Hahn-Hägerdal, K. Karhumaa, C. Fonseca, I. Spencer-Martins, M. F. Gorwa-Grauslund, Towards industrial pentose-fermenting yeast strains, App. Micro. Biotechnol., 2007,74, 5, 937-953.
A. J. A. van Maris, A. A. Winkler, M. Kuyper, W. T. A. M. de Laat, J. P. van Dijken, & J. T. Pronk, Development of efficient xylose fermentation in Saccharomyces cerevisiae: Xylose isomerase as a key component, Adv. Biochem. Engin./Biotechnol, 2007, 108, 179-204.
M. Balat, H. Balat, C. Öz, Progress in bioethanol processing. Progress in Energy and Combustion Science, 2008, 34, 5, 551-573.
C. Verduyn,. (). Physiology of yeasts in relation to biomass yields, Antonie van Leeuwenhoek, 1991, 60, 3, 325-353.
X. Q. Zhao, F. W. Bai, Mechanisms of yeast stress tolerance and its manipulation for efficient fuel ethanol production, J. Biotechnol., 2009, 144, 1, 23-30.
E. Palmqvist, B. Hahn-Hägerdal, (). Fermentation of lignocellulosic hydrolysates. I: Inhibition and detoxification. Bioresource Technology, 2000, 74, 1, 17-24.
T. D’Amore, C. J. Panchal, I. Russell, G. G. Stewart, A study of ethanol tolerance in yeast Cri. Rev. Biotechnol., 1990, 9, 4, 287-304.
D. A. Skoog, F. J. Holler, S. R. Crouch, Principles of Instrumental Analysis, Cengage Learning, 2017
Z. Movasaghi, S. Rehman,. I. U. Rehman, Raman spectroscopy of biological tissues, Appl. Spectrosc. Rev., 2007, 42, 5, 493-541.
E. Smith, G. Dent, Modern Raman Spectroscopy - A Practical Approach, Wiley, 2019
G. Socrates, Infrared and Raman Characteristic Group Frequencies: Tables and Charts, Wiley. 2001
D. L. Pavia, G. M. Lampman, G. S. Kriz, & J. R. Vyvyan, Introduction to Spectroscopy, Cengage Learning 2015
S. Nanda,A. K Dalai, J. A. Kozinski, Bioethanol from lignocellulosic biomass: Current findings determine research priorities, Renew. Sustain. Energy Rev, 2017, 63, 207–227.
A. J. Ragauskas, C. K. Williams, B. H. Davison, G. Britovsek, J. Cairney, C. A. Eckert, W.J Jr. Frederick, J.P. Hallett, D.J. Leak, C.L. Liotta, J.R Mielenz, R. Murphy, R. Templer, T.schaplinski, The path forward for biofuels and biomaterial,. Science, 2006, 311, 5760, 484-489.
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